Adenovirus-mediated inducible gene expression through tetracycline-controllable transactivator with nuclear localization signal.

@article{Yoshida1997AdenovirusmediatedIG,
  title={Adenovirus-mediated inducible gene expression through tetracycline-controllable transactivator with nuclear localization signal.},
  author={Yu Yoshida and Hirofumi Hamada},
  journal={Biochemical and biophysical research communications},
  year={1997},
  volume={230 2},
  pages={426-30}
}
Tetracycline-controllable expression vectors are widely used for inducible expression in mammalian cells. The limitation of this system is the difficulty in expressing high levels of the chimeric transactivator tTA. In this study, we demonstrate a utility of recombinant adenoviruses for the tetracycline-controllable expression system. Unexpectedly, the original tTA transactivator did not show sufficient regulation of the reporter gene expression driven by the tetracycline-responsive promoter… CONTINUE READING