Adenovirus-Mediated Bcl-Xl Gene Therapy Combined with Pronase Treatment Protects the Small Intestine from Radiation-Induced Enteritis in Mouse Model

@article{Koyama2014AdenovirusMediatedBG,
  title={Adenovirus-Mediated Bcl-Xl Gene Therapy Combined with Pronase Treatment Protects the Small Intestine from Radiation-Induced Enteritis in Mouse Model},
  author={Fumikazu Koyama and Kazuaki Uchimoto and Hisao Fujii and Hirofumi Hamada and Kazuo Ohashi and Takeo Nomi and Tadashi Nakagawa and Shinji Nakamura and Takeshi Ueda and Yoshiyuki Nakajima},
  journal={Journal of Genetic Syndromes \& Gene Therapy},
  year={2014},
  volume={5},
  pages={1-8}
}
Intestinal injury is a major side effect of radiation treatment for many malignancies. The present study investigated whether transducing Bcl-xL, a potent anti-apoptotic gene, into intestinal epithelial cells would exert protective effects against radiation-induced acute injuries. Methods: Adenoviral vectors containing the human Bcl-xL gene (AxCABclxL) or β-galactosidase gene (AxCAlacZ) driven by the CAG promoter were generated. To increase transduction efficiency into the mucosal epithelium… 

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