Adenovirus DNA replication in vitro: identification of a host factor that stimulates synthesis of the preterminal protein-dCMP complex.

@article{Nagata1982AdenovirusDR,
  title={Adenovirus DNA replication in vitro: identification of a host factor that stimulates synthesis of the preterminal protein-dCMP complex.},
  author={Kyosuke Nagata and R A Guggenheimer and Takemi Enomoto and Jack H. Lichy and Jerard Hurwitz},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1982},
  volume={79 21},
  pages={
          6438-42
        }
}
A protein factor that participates in the formation of a covalent complex between the 80,000-dalton precursor of the adenovirus (Ad) terminal protein (pTP) and 5'-dCMP has been isolated and characterized. This 47,000-dalton protein, isolated from nuclear extracts of uninfected HeLa cells, has been designated nuclear factor I. It is free of detectable DNA polymerase alpha, beta, and gamma activities. In the presence of Ad DNA-prot, the Ad-protein fraction (containing the pTP and the Ad… CONTINUE READING
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