Adenine requirement for nodulation of pea by an auxotrophic mutant of Rhizobium leguminosarum

  title={Adenine requirement for nodulation of pea by an auxotrophic mutant of Rhizobium leguminosarum},
  author={Clive E. Pankhurst and Erwin A. Schwinghamer},
  journal={Archives of Microbiology},
A non-nodulating auxotroph (L4-73) derived from an effective strain (L4) of Rhizobium leguminosarum has a growth requirement for adenine and thiamine. The auxotroph was able to infect the roots of the host plant Pisum sativum L. but formed root nodules (ineffective in nitrogen fixation) only when adenine and, to a lesser extent, thiamine were added to the plant substrate. Nodules formed in the presence of adenine were structurally abnormal, containing small cells in which infection threads… CONTINUE READING


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