Addressing accuracy and precision issues in iTRAQ quantitation.

  title={Addressing accuracy and precision issues in iTRAQ quantitation.},
  author={Natasha A. Karp and Wolfgang Huber and Pawel G Sadowski and Philip D. Charles and Svenja V Hester and Kathryn S. Lilley},
  journal={Molecular & cellular proteomics : MCP},
  volume={9 9},
iTRAQ (isobaric tags for relative or absolute quantitation) is a mass spectrometry technology that allows quantitative comparison of protein abundance by measuring peak intensities of reporter ions released from iTRAQ-tagged peptides by fragmentation during MS/MS. However, current data analysis techniques for iTRAQ struggle to report reliable relative protein abundance estimates and suffer with problems of precision and accuracy. The precision of the data is affected by variance heterogeneity… CONTINUE READING
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