• Corpus ID: 21759616

Additive effects of steel factor and antisense TGF-beta 1 oligodeoxynucleotide on CD34+ hematopoietic progenitor cells.

@article{Li1994AdditiveEO,
  title={Additive effects of steel factor and antisense TGF-beta 1 oligodeoxynucleotide on CD34+ hematopoietic progenitor cells.},
  author={M L Li and Angelo A. Cardoso and P. Sansilvestri and Antoinette Hatzfeld and Eugene L. Brown and Hemchand Sookdeo and Jean-Pierre L{\'e}vesque and Steven C. Clark and Jacques A. Hatzfeld},
  journal={Leukemia},
  year={1994},
  volume={8 3},
  pages={
          441-5
        }
}
We previously demonstrated that TGF-beta 1 antisense oligodeoxynucleotides can release early CD34+ bone marrow progenitors from quiescence, and increase the numbers of mixed and large erythroid colonies. As Steel Factor (SF) has a similar effect on colony formation by CD34+ cells, we tested whether this factor acts by blocking the inhibitory effects of TGF-beta. That this is not generally the case was demonstrated by the finding that the combination of TGF-beta 1 antisense with SF in cultures… 
Inhibition of autocrine TGF-ß in CD34+ human progenitor cells reveals their potentiality to engraft adults and improves gene transfer efficiency
TLDR
It is shown that the capacity for long-term engraftment of a 100-200 ml sample of umbilical cord blood is greater than that of the volume of bone marrow typically used to engraft an adult and that inclusion of SF increases the frequency of colony formation by early progenitor cells, but it still does not reverse the effects of TGF-81.
Increased stable retroviral gene transfer in early hematopoietic progenitors released from quiescence.
TLDR
Prestimulation with cytokine prestimulation of SBA-CD34high human umbilical cord blood progenitors provides a substantial improvement for gene transfer efficiency within the quiescent stem cell compartment that is responsible for long-term engraftment.
TGF-(beta)1 maintains hematopoietic immaturity by a reversible negative control of cell cycle and induces CD34 antigen up-modulation.
TLDR
It is demonstrated that low, physiological concentrations of TGF-(beta)1, which specifically maintain primitive human hemopoietic stem/progenitor cells in quiescence, have a reversible effect and do not induce apoptosis, providing additional evidence that TGF-beta1 acts as a key physiological factor ensuring the maintenance of a stem cell reserve.
High proliferative potential-quiescent cells: a working model to study primitive quiescent hematopoietic cells.
TLDR
The working model of 'High Proliferative Potential-Quiescent cells' is proposed to refer to these primitive hematopoietic multipotent progenitors that are highly sensitive to the growth inhibitory effect of TGF-beta1.
Molecular mechanism of transforming growth factor beta-mediated cell-cycle modulation in primary human CD34(+) progenitors.
The mechanisms by which transforming growth factor beta (TGF-beta) exerts a negative effect on cell-cycle entry in primary human hematopoietic stem/progenitor cells were examined at the molecular and
Ex vivo expansion of CD34 + CD38- cord blood cells.
CD34+ cord blood (CB) cells were expanded in stromal cell-free long-term culture (LTC), in the presence of various combinations of interleukin-3 (IL-3), stem cell factor (SCF), IL-6,
Early human thymocyte proliferation is regulated by an externally controlled autocrine transforming growth factor-beta 1 mechanism.
TLDR
A role for TGF beta 1 is suggested as an externally controlled, autocrine inhibitory factor for human early thymocytes, with a regulatory role in thymic T-cell output.
Biologic and phenotypic analysis of early hematopoietic progenitor cells in umbilical cord blood
TLDR
Umbilical cord blood is a source of progenitor cells with immature characteristics in terms of surface antigen expression, distribution of SCF receptor, resistance to mafosfamide and replating potential, and represents an ideal candidate population for experimental programs involving gene transfer and ex vivo stem cell expansion.
AN INCREASE IN THE LEVELS OF RETROVIRAL-MEDIATED TRANSDUCTION OF ENGRAFTING HUMAN HEMATOPOIETIC PROGENITORS CAN BE OBTAINED BY MANIPULATION OF THE HEMATOPOIETIC CELL CYCLE
TLDR
The bnx/hu xenograft model can be used to assess the effects of various in vitro trans- duction methods on the efficacy of gene transfer, as well as on the viability and commitment to differentiation of the target human hematopoietic stem and progenitor cells.
Differential activation of MAPK signaling pathways by TGF-beta1 forms the molecular mechanism behind its dose-dependent bidirectional effects on hematopoiesis.
  • V. Kale
  • Biology, Medicine
    Stem cells and development
  • 2004
TLDR
TGF-beta1 at low doses (picogram levels) stimulated the colony formation from CD34+ cells, indicating that these progenitors form the direct target of stimulatory action of TGF- beta1.
...
1
2
3
...