Additional TCRV beta primers and minor method modifications improve detection of clonal T-cell populations by RT-PCR.

Abstract

The TCRV beta RT-PCR method for detection of clonal populations of T cells which we described previously could not detect clones that used certain variable (V) beta region families. V beta 2, 4, 8.3, and 18 had insufficient homology with the original consensus V region primer. Two new primers have been designed which work well and are able to amplify from V… (More)

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