Addition of genes for cellobiase and pectinolytic activity in Escherichia coli for fuel ethanol production from pectin-rich lignocellulosic biomass.

@article{Edwards2011AdditionOG,
  title={Addition of genes for cellobiase and pectinolytic activity in Escherichia coli for fuel ethanol production from pectin-rich lignocellulosic biomass.},
  author={Meredith C. Edwards and Emily Decrescenzo Henriksen and Lorraine P. Yomano and Brian C Gardner and Lekh N Sharma and Lonnie O'Neal Ingram and Joy Doran Peterson},
  journal={Applied and environmental microbiology},
  year={2011},
  volume={77 15},
  pages={5184-91}
}
Ethanologenic Escherichia coli strain KO11 was sequentially engineered to contain the Klebsiella oxytoca cellobiose phosphotransferase genes (casAB) as well as a pectate lyase (pelE) from Erwinia chrysanthemi, yielding strains LY40A (casAB) and JP07 (casAB pelE), respectively. To obtain an effective secretion of PelE, the Sec-dependent pathway out genes from E. chrysanthemi were provided on a cosmid to strain JP07 to construct strain JP07C. Finally, oligogalacturonide lyase (ogl) from E… CONTINUE READING

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Lignocellulose Conversion

Springer Berlin Heidelberg • 2013

References

Publications referenced by this paper.
Showing 1-10 of 31 references

Molecular cloning: a laboratory manual, 2nd ed

J. Sambrook, E. F. Fritsch, T. Maniatis
1989
View 14 Excerpts
Highly Influenced

How biotech can transform biofuels

Nature Biotechnology • 2008
View 1 Excerpt

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