Addition of destabilizing poly (A)-rich sequences to endonuclease cleavage sites during the degradation of chloroplast mRNA.

@article{Lisitsky1996AdditionOD,
  title={Addition of destabilizing poly (A)-rich sequences to endonuclease cleavage sites during the degradation of chloroplast mRNA.},
  author={I. Lisitsky and Petra Klaff and G. Schuster},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1996},
  volume={93 23},
  pages={13398-403}
}
In this work, we report the posttranscriptional addition of poly(A)-rich sequences to mRNA in chloroplasts of higher plants. Several sites in the coding region and the mature end of spinach chloroplast psbA mRNA, which encodes the D1 protein of photosystem II, are detected as polyadenylylated sites. In eukaryotic cells, the addition of multiple adenosine residues to the 3' end of nuclear RNA plays a key role in generating functional mRNAs and in regulating mRNA degradation. In bacteria, the… CONTINUE READING

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