AddTag, a two-step approach with supporting software package that facilitates CRISPR/Cas-mediated precision genome editing

@article{Seher2021AddTagAT,
  title={AddTag, a two-step approach with supporting software package that facilitates CRISPR/Cas-mediated precision genome editing},
  author={Thaddeus D Seher and Namkha Nguyen and Diana Ramos and Priya Bapat and Clarissa J. Nobile and Suzanne S. Sindi and Aaron D Hernday},
  journal={G3: Genes|Genomes|Genetics},
  year={2021},
  volume={11}
}
Abstract CRISPR/Cas-induced genome editing is a powerful tool for genetic engineering, however, targeting constraints limit which loci are editable with this method. Since the length of a DNA sequence impacts the likelihood it overlaps a unique target site, precision editing of small genomic features with CRISPR/Cas remains an obstacle. We introduce a two-step genome editing strategy that virtually eliminates CRISPR/Cas targeting constraints and facilitates precision genome editing of elements… 
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