Adaptor protein complexes-1 and 3 are involved at distinct stages of flavivirus life-cycle


Intracellular protein trafficking pathways are hijacked by viruses at various stages of viral life-cycle. Heterotetrameric adaptor protein complexes (APs) mediate vesicular trafficking at distinct intracellular sites and are essential for maintaining the organellar homeostasis. In the present study, we studied the effect of AP-1 and AP-3 deficiency on flavivirus infection in cells functionally lacking these proteins. We show that AP-1 and AP-3 participate in flavivirus life-cycle at distinct stages. AP-3-deficient cells showed delay in initiation of Japanese encephalitis virus and dengue virus RNA replication, which resulted in reduction of infectious virus production. AP-3 was found to colocalize with RNA replication compartments in infected wild-type cells. AP-1 deficiency affected later stages of dengue virus infection where increased intracellular accumulation of infectious virus was observed. Therefore, our results propose a novel role for AP-1 and AP-3 at distinct stages of infection of some of the RNA viruses.

DOI: 10.1038/srep01813

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@inproceedings{Agrawal2013AdaptorPC, title={Adaptor protein complexes-1 and 3 are involved at distinct stages of flavivirus life-cycle}, author={Tanvi Agrawal and Peter Schu and Guruprasad R Medigeshi}, booktitle={Scientific reports}, year={2013} }