Adaptive optics in multiphoton microscopy: comparison of two, three and four photon fluorescence.

  title={Adaptive optics in multiphoton microscopy: comparison of two, three and four photon fluorescence.},
  author={David Sinefeld and Hari P. Paudel and Dimitre G. Ouzounov and T. G. Bifano and Chris Xu},
  journal={Optics express},
  volume={23 24},
We demonstrate adaptive optics system based on nonlinear feedback from 3- and 4-photon fluorescence. The system is based on femtosecond pulses created by soliton self-frequency shift of a 1550-nm fiber-based femtosecond laser together with micro-electro-mechanical system (MEMS) phase spatial light modulator (SLM). We perturb the 1020-segment SLM using an orthogonal Walsh sequence basis set with a modified version of three-point phase shifting interferometry. We show the improvement after… CONTINUE READING


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