Adaption of neutral protease activity for islet isolation from the long-term two-layer method-stored pig pancreas.

Abstract

BACKGROUND Islet release from the pancreas is mediated by both collagenase and neutral protease (NP), a critical effector of islet integrity. To prove the hypothesis that adjustment of NP reduces islet damage after prolonged ischemia, adult pig pancreata were digested after 7-hour preservation by the two-layer method (TLM) using a 2-component enzyme blend… (More)

Topics

Cite this paper

@article{Brandhorst2005AdaptionON, title={Adaption of neutral protease activity for islet isolation from the long-term two-layer method-stored pig pancreas.}, author={Daniel Brandhorst and Markus Iken and Mathias D. Brendel and Reinhard Georg Bretzel and Heide Brandhorst}, journal={Transplantation proceedings}, year={2005}, volume={37 1}, pages={458-9} }