Adapting the Smart-seq2 Protocol for Robust Single Worm RNA-seq.

@article{Serra2018AdaptingTS,
  title={Adapting the Smart-seq2 Protocol for Robust Single Worm RNA-seq.},
  author={Lorrayne Serra and Dennis Chang and Marissa G. Macchietto and Katherine Williams and Rabi Murad and Dihong Lu and Adler R Dillman and Ali Mortazavi},
  journal={Bio-protocol},
  year={2018},
  volume={8 4}
}
Most nematodes are small worms that lack enough RNA for regular RNA-seq protocols without pooling hundred to thousand of individuals. We have adapted the Smart-seq2 protocol in order to sequence the transcriptome of an individual worm. While developed for individual Steinernema carpocapsae and Caenorhabditis elegans larvae as well as embryos, the protocol should be adaptable for other nematode species and small invertebrates. In addition, we describe how to analyze the RNA-seq results using the… CONTINUE READING

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