Adaptation to non-ammoniagenic medium and selective substrate feeding lead to enhanced yields in animal cell cultures.

@article{Hassell1990AdaptationTN,
  title={Adaptation to non-ammoniagenic medium and selective substrate feeding lead to enhanced yields in animal cell cultures.},
  author={Thomas Hassell and Michael L. Butler},
  journal={Journal of cell science},
  year={1990},
  volume={96 ( Pt 3)},
  pages={
          501-8
        }
}
Methods for the adaptation of three animal cell lines to media in which glutamine is replaced with either glutamate or 2-oxoglutarate are described. The cell lines differ in their rate of adaptation. The consequences of the adaptation of the McCoy cell line to a glutamate-based medium was measured in terms of: cell yield (increased by 17%), ammonia accumulation (reduced by 70%), glucose consumption (decreased by greater than 70%) and lactate accumulation (decreased by greater than 75%). The… CONTINUE READING

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References

Publications referenced by this paper.
SHOWING 1-10 OF 28 REFERENCES

The effect of metabolic by - products on animal cells in culture

H. U. Bergmeyer, T. HASSELL, C DOYLE, S. GLEAVE, P. JENNINGS
  • Proc . Eur . Soc . Animal Cell Tech . Meeting , Avignon ( in press )
  • 1990

Effects on cell proliferation of metabolites produced by cultured cells and their removal from culture in denned media

M Iio, A. MORIYAMA, R. MURAKAMI
  • In Proc. Int. Symp. on Growth and Differentiation of Cells in Defined Environment
  • 1984
VIEW 1 EXCERPT