Adaptation of the CHARM DNA methylation platform for the rat genome reveals novel brain region-specific differences.

@article{Lee2011AdaptationOT,
  title={Adaptation of the CHARM DNA methylation platform for the rat genome reveals novel brain region-specific differences.},
  author={Richard Y. Lee and Kellie L. K. Tamashiro and Martin J. Aryee and Peter N. Murakami and Fayaz Seifuddin and Brian R. Herb and Yuqing Huo and Michael A Rongione and Andrew P. Feinberg and Timothy H. Moran and James B. Potash},
  journal={Epigenetics},
  year={2011},
  volume={6 11},
  pages={1378-90}
}
Comprehensive High-throughput Arrays for Relative Methylation (CHARM) was recently developed as an experimental platform and analytic approach to assess DNA methylation (DNAm) at a genome-wide level. Its initial implementation was for human and mouse. We adapted it for rat and sought to examine DNAm differences across tissues and brain regions in this model organism. We extracted DNA from liver, spleen, and three brain regions: cortex, hippocampus, and hypothalamus from adult Sprague Dawley… CONTINUE READING