Adaptation of a CCR5-using, primary human immunodeficiency virus type 1 isolate for CD4-independent replication.

@article{Kolchinsky1999AdaptationOA,
  title={Adaptation of a CCR5-using, primary human immunodeficiency virus type 1 isolate for CD4-independent replication.},
  author={Peter Kolchinsky and Tajib A. Mirzabekov and Michael Farzan and Enko N. Kiprilov and Mark Cayabyab and Larissa J Mooney and Hyeryun Choe and Joseph Sodroski},
  journal={Journal of virology},
  year={1999},
  volume={73 10},
  pages={8120-6}
}
The gp120 envelope glycoprotein of the human immunodeficiency virus type 1 (HIV-1) promotes virus entry by sequentially binding CD4 and chemokine receptors on the target cell. Primary, clinical HIV-1 isolates require interaction with CD4 to allow gp120 to bind the CCR5 chemokine receptor efficiently. We adapted a primary HIV-1 isolate, ADA, to replicate in CD4-negative canine cells expressing human CCR5. The gp120 changes responsible for the adaptation were limited to alteration of… CONTINUE READING

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