Acute multidrug delivery via a wearable bioreactor facilitates long-term limb regeneration and functional recovery in adult Xenopus laevis

  title={Acute multidrug delivery via a wearable bioreactor facilitates long-term limb regeneration and functional recovery in adult Xenopus laevis},
  author={Nirosha J Murugan and Hannah J Vigran and Kelsie A. Miller and Annie Golding and Quang L Pham and Megan M. Sperry and Cody Rasmussen-Ivey and Anna Kane and David L. Kaplan and Michael Levin},
  journal={Science Advances},
Limb regeneration is a frontier in biomedical science. Identifying triggers of innate morphogenetic responses in vivo to induce the growth of healthy patterned tissue would address the needs of millions of patients, from diabetics to victims of trauma. Organisms such as Xenopus laevis—whose limited regenerative capacities in adulthood mirror those of humans—are important models with which to test interventions that can restore form and function. Here, we demonstrate long-term (18 months… 

Cellular responses in the FGF10‐mediated improvement of hindlimb regenerative capacity in Xenopus laevis revealed by single‐cell transcriptomics

Cell clusters exhibiting a differential gene expression pattern compared with that in controls treated with phosphate‐buffered saline‐treatment conditions are explored, suggesting a possible role of these clusters in improving the regenerative capacity of hindlimbs via FGF10 administration.

Back to pluripotency: fully chemically induced reboot of human somatic cells

An exciting strategy to reprogram human somatic cells into human chemically induced pluripotent stem cells (hCiPSCs) is shown, identifying crucial steps and pathways that pose a barrier to chemically induced reprogramming of human cells.

Mammalian organ regeneration in spiny mice.

The evidence that Acomys represents an emerging model organism that offers a unique opportunity for the biomedical community to investigate and clinically translate molecular mechanisms of scarless wound healing and regeneration of organ function in a mammalian species is discussed.



Review: Limb regeneration in humans: Dream or reality?

  • L. Alibardi
  • Biology
    Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft
  • 2018

Tail Regeneration in Xenopus laevis as a Model for Understanding Tissue Repair

What is known about the chemical and bioelectric signals underlying this process are reviewed and analogies to evolutionarily conserved pathways in other patterning systems are drawn.

Digit Tip Regeneration: Merging Regeneration Biology with Regenerative Medicine

It is proposed that regeneration‐incompetent injuries possess dormant regenerative potential that can be activated by targeted treatment with specific morphogenetic agents, and it is believed that future Regenerative Medicine‐based‐therapies should be designed to promote, not replace, regenerative responses.

Comparative regenerative mechanisms across different mammalian tissues

Recent insights in the elucidation of molecular mechanisms of regeneration that have come from studies of tissue homeostasis and injury repair in mammalian tissues that span the spectrum from little or no self-renewal to those showing active cell turnover throughout life are considered.

BMP signaling induces digit regeneration in neonatal mice

The evidence suggests that BMP treatment triggers a reprogramming event that re-initiates digit tip development at the amputation wound, and demonstrates for the first time that the postnatal mammalian digit has latent regeneration capabilities that can be induced by growth factor treatment.

Lgr6 marks nail stem cells and is required for digit tip regeneration

This work identifies a cell population expressing a mediator of Wnt signaling, Lgr6 (leucine-rich repeat-containing G protein-coupled receptor 6), as key stem cells for the nail, and finds that Lgr7 is required for proper digit tip regeneration, and reports on two important agonists of the Wnt pathway that are known to be markers of several epithelial stem cell populations.