1756-4646/$ see front matter 2013 Elsevi http://dx.doi.org/10.1016/j.jff.2013.04.015 * Corresponding authors. Tel.: +82 2 970 6740 E-mail addresses: email@example.com Mulberry leaf extract was investigated as a potent alpha-glucosidase inhibitor, especially for individuals with prediabetes. We evaluated the effect of the ingestion of mulberry leaf aqueous extract (MLAE) on postprandial glucose responses in healthy subjects. We carried out a randomized, double-blind, placebo-controlled trial to determine the intake amount of MLAE (1.25, 2.5, or 5 g) and time necessary to impact postprandial blood glucose levels after 75 g of maltose loading in 50 healthy subjects. Ingestion of MLAE led to a decrease in postchallenge acute glucose levels following intake of 2.5 or 5 g of MLAE at 30 and 60 min (P = 0.0137 and 0.0423, respectively). There was no significant difference between preand simultaneous administration of MLAE. The ingestion of MLAE resulted in improved postprandial glycemic control in healthy subjects. 2013 Elsevier Ltd. All rights reserved.