Acute inactivation of the Aspergillus nidulans Golgi membrane fusion machinery: correlation of apical extension arrest and tip swelling with cisternal disorganization.

@article{Pinar2013AcuteIO,
  title={Acute inactivation of the Aspergillus nidulans Golgi membrane fusion machinery: correlation of apical extension arrest and tip swelling with cisternal disorganization.},
  author={Mario Pinar and Areti Pantazopoulou and Herbert N. Arst and Miguel A Pe{\~n}alva},
  journal={Molecular microbiology},
  year={2013},
  volume={89 2},
  pages={228-48}
}
The mechanisms governing traffic across the Golgi are incompletely understood. We studied, by live-cell microscopy, the consequences of disorganizing the Aspergillus nidulans Golgi, using an extended set of fluorescent protein markers to resolve early from late cisternae. The early Golgi syntaxin SedV(Sed) (5) and the RabO(Rab) (1) regulatory GTPase play essential roles in secretion, cooperating in the ER-Golgi interface. Following a temperature shift-up 'on-the-stage', hyphae carrying… CONTINUE READING

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