Acute hyperoxia-induced transcriptional response in the mouse RPE/choroid.

  title={Acute hyperoxia-induced transcriptional response in the mouse RPE/choroid.},
  author={Andrea L Weigel and Hisashi Ida and Sharon A. Boylan and Leonard M. Hjelmeland},
  journal={Free radical biology & medicine},
  volume={35 5},
Oxidative stress has been studied in the retinal pigmented epithelium (RPE) in vitro but not in vivo. Our purpose, therefore, was to develop an in vivo model of acute oxidative stress in the C57BL/6J mouse. Mice were exposed to > or = 98% oxygen for 0, 2, or 6 h, and amplified total RNA from the RPE/choroid was applied to microarrays examining about 2200 unique genes. Statistical analysis determined that 642 genes, out of a total of 1349 expressed, were significantly downregulated at only 2 h… CONTINUE READING