Acute endotoxemia in rats induces down-regulation of V2 vasopressin receptors and aquaporin-2 content in the kidney medulla.

@article{Grinevich2004AcuteEI,
  title={Acute endotoxemia in rats induces down-regulation of V2 vasopressin receptors and aquaporin-2 content in the kidney medulla.},
  author={Valery Grinevich and Mark A. Knepper and Joseph G. Verbalis and Ivan Reyes and G. Romero Aguilera},
  journal={Kidney international},
  year={2004},
  volume={65 1},
  pages={54-62}
}
BACKGROUND Endotoxemia can lead to fluid metabolism alterations despite unchanged or elevated plasma vasopressin (VP) levels, suggesting a refractoriness of the kidney to the effect of the peptide. To test this hypothesis, we examined the effect of lipopolysaccharide (LPS) injection on the expression of V2 receptors and aquaporin-2 in the kidney. METHODS Plasma VP and urine osmolality, and binding of [3H]VP to kidney membranes, Western blot, and immunohistochemical analysis of aquaporin-2, in… CONTINUE READING