Acute dietary nitrate supplementation improves arterial endothelial function at high altitude: A double-blinded randomized controlled cross over study.

Abstract

INTRODUCTION Dietary nitrate (NO3(-)) supplementation serves as an exogenous source of nitrite (NO2(-)) and nitric oxide (NO) through the NO3(-) - NO2(-) - NO pathway, and may improve vascular functions during normoxia. The effects of NO3(-) supplementation in healthy lowlanders during hypobaric hypoxia are unknown. PURPOSE Determine the effect of acute oral NO3(-)supplementation via beetroot juice (BJ) on endothelial function (flow mediated dilation; FMD) in lowlanders at 3700 m. METHODS FMD was measured using ultrasound and Doppler in the brachial artery of 11 healthy subjects (4 females, age 25 ± 5 yrs; height 1.8 ± 0.1 m, weight 72 ± 10 kg) sojourning to high altitude. In a randomized, double-blinded crossover study design, FMD was measured 3 h after drinking BJ (5.0 mmol NO3(-)) and placebo (PL; 0.003 mmol NO3(-)) supplementation at 3700 m, with a 24-h wash out period between tests. FMD was also measured without any BJ supplementation pre-trek at 1370 m, after 5 days at 4200 m and upon return to 1370 m after 4 weeks of altitude exposure (above 2500 m). The altitude exposure was interrupted by a decent to lower altitude where subjects spent two nights at 1370 m before returning to altitude again. RESULTS Ten subjects completed the NO3(-) supplementation. FMD (mean ± SD) pre-trek value was 6.53 ± 2.32% at 1370 m. At 3700 m FMD was reduced to 3.84 ± 1.31% (p < 0.01) after PL supplementation but was normalized after receiving BJ (5.77 ± 1.14% (p = 1.00). Eight of the subjects completed the interrupted 4-week altitude stay, and their FMD was lower at 4200 m (FMD 3.04 ± 2.22%) and at post-altitude exposure to 1370 m (FMD 3.91 ± 2.58%) compared to pre-trek FMD at 1370 m. CONCLUSION Acute dietary NO3(-)supplementation may abolish altitude-induced reduction in endothelial function, and can serve as a dietary strategy to ensure peripheral vascular function in lowland subjects entering high altitude environments.

DOI: 10.1016/j.niox.2015.08.006
02040608020162017
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@article{Bakker2015AcuteDN, title={Acute dietary nitrate supplementation improves arterial endothelial function at high altitude: A double-blinded randomized controlled cross over study.}, author={Emily Bakker and Harald K Engan and Alexander Patrician and Erika K A Schagatay and Trine Karlsen and Ulrik Wisl\off and Svein Erik Gaustad}, journal={Nitric oxide : biology and chemistry}, year={2015}, volume={50}, pages={58-64} }