Acute Exercise Stimulates Carnitine Biosynthesis and OCTN2 Expression in Mouse Kidney

  title={Acute Exercise Stimulates Carnitine Biosynthesis and OCTN2 Expression in Mouse Kidney},
  author={Tom L Broderick and Frank A Cusimano and Chelsea Carlson and Leslie K. Tamura},
  journal={Kidney and Blood Pressure Research},
  pages={398 - 405}
Background/Aims: Carnitine is essential for the transport of long-chain FAs (FA) into the mitochondria for energy production. During acute exercise, the increased demand for FAs results in a state of free carnitine deficiency in plasma. The role of kidney in carnitine homeostasis after exercise is not known. Methods: Swiss Webster mice were sacrificed immediately after a 1-hour moderate intensity treadmill run, and at 4-hours and 8-hours into recovery. Non-exercising mice served as controls… Expand
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Effects of Exercise Training on Renal Carnitine Biosynthesis and Uptake in the High-Fat and High-Sugar-Fed Mouse
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Effects of Dietary Anaplerotic and Ketogenic Energy Sources on Renal Fatty Acid Oxidation Induced by Clofibrate in Suckling Neonatal Pigs
  • X. Lin, B. Pike, +5 authors J. Odle
  • Medicine, Chemistry
  • International journal of molecular sciences
  • 2020
Modification of anaplerosis or ketogenesis via dietary substrates had no influence on in vitro renal palmitic acid oxidation induced by PPARα activation. Expand
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Age and substrate affect γ-BBH specific activity and Km in liver and kidney and while the predominant organ for carnitine synthesis is likely the kidney at birth, the liver appears to predominate after the pig exceeds 7 day of age. Expand
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Military training-related acute kidney injury
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The effects of exercise training on γ-butyrobetaine hydroxylase and novel organic cation transporter-2 gene expression in the rat.
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L-carnitine attenuates the development of kidney fibrosis in hypertensive rats by upregulating PPAR-γ.
This study confirms the efficacy of L- carnitine against hypertension-associated renal fibrosis from in vivo and in vitro studies and suggests that the L-c Carnitine effect occurs in a PPAR-γ-dependent manner. Expand
Influence of carnitine supplementation on muscle substrate and carnitine metabolism during exercise.
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PPAR alpha-activation results in enhanced carnitine biosynthesis and OCTN2-mediated hepatic carnitine accumulation.
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Kinetics of carnitine‐dependent fatty acid oxidation
Analysis of homogenates prepared from skeletal muscle, liver, kidney, and heart of the rat, and from canine and human skeletal muscle provides a plausible explanation for the fact that muscle fatty acid metabolism is severely impaired in the syndrome of human carnitine deficiency. Expand
Fasting and Caloric Restriction Increases mRNA Concentrations of Novel Organic Cation Transporter-2 and Carnitine Concentrations in Rat Tissues
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[Carnitine biosynthesis in mammals].
  • A. Bach
  • Medicine
  • Reproduction, nutrition, developpement
  • 1982
It appears that carnitine synthesis is not slowed down by prolonged fasting, that itdoes not completely cover body needs during the first postnatal days, and that it does not decrease in two patients with systematic carnitines deficiency. Expand
Regulation of Octn2 transporter (SLC22A5) by peroxisome proliferator activated receptor alpha.
The results indicate that the nuclear receptor PPARalpha directly up-regulates the expression of rOctn2 and increases the hepatic uptake of carnitine via r Octn2. Expand
Molecular and Functional Characterization of Organic Cation/Carnitine Transporter Family in Mice*
OCTN3 is unique in its limited tissue distribution and Na+-independent carnitine transport, whereas OCTN1 efficiently transported TEA with minimal expression of carn itine transport activity and may have a different role from other members of the OCTN family. Expand