Activity of the H2-oxidizing hydrogenase in different N2-fixing bacteria

  title={Activity of the H2-oxidizing hydrogenase in different N2-fixing bacteria},
  author={M. Pinkwart and H. Bahl and M. Reimer and D. W{\"o}lfle and H. Berndt},
  journal={Fems Microbiology Letters},
The Genus Xanthobacter
Nitrogen fixation by the hydrogen-oxidizing bacterium Alcaligenes latus
The aerobic hydrogen-oxidizing bacterium Alcaligenes latus represented by three strains was found to be able to grow with dinitrogen as the sole nitrogen source: The doubling time of total (Kjeldahl)Expand
Production of molecular hydrogen in microorganisms
This review surveys recent data on microorganisms able to evolve molecular hydrogen, conditions of H2 production and properties of enzymes catalyzing this process. The data are discussed with regardExpand
Influences of pH, Temperature, and Moisture on Gaseous Tritium Uptake in Surface Soils
  • R. Fallon
  • Chemistry, Medicine
  • Applied and environmental microbiology
  • 1982
Soil uptake of T(2)-HT was not severely limited by pH, temperature, or moisture in the soils tested, and rapid exchange of gaseous tritium into soil water must be expected and accounted for in modeling the isotope distributions around nuclear facilities. Expand


Determination of Hydrogenase in Free-living Cultures of Rhizobium japonicum and Energy Efficiency of Soybean Nodules.
  • S. T. Lim
  • Biology, Medicine
  • Plant physiology
  • 1978
The tritium exchange assay provides an easy, quick, and accurate assessment of H(2) uptake efficiency of intact nodules of Rhizobium japonicum. Expand
Differentiation of Rhizobium japonicum, III. Inhibition of Nitrogenase Derepression by Chloramphenicol and Rifampicin Concentrations, Not Inhibiting Growth
  • D. Werner
  • Biology, Medicine
  • Zeitschrift fur Naturforschung. Section C, Biosciences
  • 1978
Development of nitrogenase (40 -140 nmol C2 H4 · mg protein-1· h-1) in Rhizobium japonicum 61-A-101 after transfer to special culture conditions is inhibited by chloramphenicol and rifampicin, but activity of previously derepressed cells is not inhibited. Expand
Expression of hydrogenase activity in free-living Rhizobium japonicum.
A spontaneous R. japonicum mutant strain is isolated that forms nodules that evolve H(2) but other symbiotic characteristics appear normal and will be useful in evaluating the importance of the hydrogenase system in the nitrogen-fixing process of legumes. Expand
Hydrogen-dependent growth of Escherichia coli in anaerobic respiration and the presence of hydrogenases with different functions.
E. coli K10 was found to grow anaerobically on molecular hydrogen by reducing nitrate, fumarate, and trimethylamine N-oxide when peptone was added to the culture medium, suggesting that multiple forms of hydrogenase are present. Expand
Hydrogenase activity in Paracoccus denitrificans. Partial purification and interaction with the electron transport chain.
Ceci indique que l'hydrogenase peut reduire directement le benzyl viologene, mais pas les cellules intactes, catalysent the reduction du ferricyanure de potassium par H2. Expand
Mutant strains of clover rhizobium (Rhizobium trifolii) that form nodules on soybean (Glycine max).
  • F. O'Gara, K. Shanmugam
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1978
Mutant strains of Rhizobium trifolii that induce nitrogenase activity in the free-living state were isolated. These strains, unlike the wild type, nodulated soybean and mungbean plants, producingExpand
Properties of the hydrogenase system in Rhizobium japonicum bacteroids.
The addition of hydrogen to bacteroids increases oxygen uptake but decreases respiratory CO2 production, indicating a conservation of endogenous substrates. Expand
An inducible hydrogenase in cyanobacteria enhances N2 fixation
Two questions have been examined: first, whether hydrogenase in N. muscorum and A. cylindrica is an inducible enzyme and, secondly, whether there is a cooperativity between the uptake of Hz catalyzed by hydrogenase and the rate and efficiency of N*-uptake catalyzing by nitrogenase in aerobically and Hz-grown cultures of these microorganisms. Expand
H2 metabolism in the photosynthetic bacterium Rhodopseudomonas capsulata: production and utilization of H2 by resting cells
The photoreduction activity of cells grown on lactate + glutamate (which contain active nitrogenase) is greatly activated by NH4+, but this effect is not observed in cells grown with NH4+ as N source (nitrogenase repressed) or in a Nif- mutant that is unable to produce H2. Expand