Activity of prostaglandin biosynthetic pathways in rat pancreatic islets.

@article{Kelly1984ActivityOP,
  title={Activity of prostaglandin biosynthetic pathways in rat pancreatic islets.},
  author={Kathleen L. Kelly and Suzanne Gale Laychock},
  journal={Prostaglandins},
  year={1984},
  volume={27 6},
  pages={
          925-38
        }
}
Isolated pancreatic islets of the rat were either prelabeled with [3H]arachidonic acid, or were incubated over the short term with the concomitant addition of radiolabeled arachidonic acid and a stimulatory concentration of glucose (17mM) for prostaglandin (PG) analysis. In prelabeled islets, radiolabel in 6-keto-PGF1 alpha, PGE2, and 15-keto-13,14-dihydro-PGF2 alpha increased in response to a 5 min glucose (17mM) challenge. In islets not prelabeled with arachidonic acid, label incorporation in… CONTINUE READING
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