Activity of microbial lipase frompénicillium speciesin vitro andin vivo

Abstract

The lipase activity was determined as follows. To 2.5 ml of the olive oil emulsion we added 2 ml of phosphate buffer solution and mixed in a constant-temperature bath at 37~ for 20 min. Then 0.5 ml of the enzyme, dissolved in buffer solution, was added to the contents of the flask. The mixture obtained was mixed, again placed in the constant-temperature… (More)
DOI: 10.1007/BF00779083

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6 Figures and Tables