Activity of a recombinant fusion protein between transforming growth factor type alpha and Pseudomonas toxin.

@article{Chaudhary1987ActivityOA,
  title={Activity of a recombinant fusion protein between transforming growth factor type alpha and Pseudomonas toxin.},
  author={Vijay K Chaudhary and David J P Fitzgerald and Sankar 8 Adhya and Ira Pastan},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1987},
  volume={84 13},
  pages={4538-42}
}
The transforming growth factor type alpha gene has been fused to a modified Pseudomonas toxin gene from which the cell-recognition domain has been deleted. The chimeric gene has been expressed in Escherichia coli, and the chimeric protein, PE40-TGF-alpha, has been highly purified. PE40-TGF-alpha kills cells expressing epidermal growth factor receptors and has little activity against cells with few receptors. This chimeric protein might be useful in treating cancers that contain high numbers of… CONTINUE READING