Activity-based protein profiling implicates urokinase activation as a key step in human fibrosarcoma intravasation.

@article{Madsen2006ActivitybasedPP,
  title={Activity-based protein profiling implicates urokinase activation as a key step in human fibrosarcoma intravasation.},
  author={Mark A Madsen and Elena I. Deryugina and Sherry M Niessen and Benjamin F Cravatt and James P. Quigley},
  journal={The Journal of biological chemistry},
  year={2006},
  volume={281 23},
  pages={15997-6005}
}
Entry of malignant cells into the vasculature (i.e. intravasation) requires proteolytic remodeling of the extracellular matrix so that tumor cells may pass through the local stroma and penetrate the vessel wall. The circulatory system then provides a means of transporting tumor cells to distant sites where they extravasate and establish metastatic lesions. This study utilizes activity-based protein profiling to compare the active serine hydrolase repertoire in high intravasating (HT-hi/diss… CONTINUE READING