Activity-based mass spectrometric characterization of proteases and inhibitors in human saliva.

@article{Sun2009ActivitybasedMS,
  title={Activity-based mass spectrometric characterization of proteases and inhibitors in human saliva.},
  author={Xiuli Sun and Erdjan Salih and Frank G. Oppenheim and E J Helmerhorst},
  journal={Proteomics. Clinical applications},
  year={2009},
  volume={3 7},
  pages={810-820}
}
Proteases present in oral fluid effectively modulate the structure and function of some salivary proteins and have been implicated in tissue destruction in oral disease. To identify the proteases operating in the oral environment, proteins in pooled whole saliva supernatant were separated by anion-exchange chromatography and individual fractions were analyzed for proteolytic activity by zymography using salivary histatins as the enzyme substrates. Protein bands displaying proteolytic activity… CONTINUE READING

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