Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination.

@article{Li2013ActivityAR,
  title={Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination.},
  author={Yunfeng Li and Xuan Yi Butzin and Andrew Davis and Barbara Setlow and George Korza and Fatma Işik {\"U}stok and Graham Christie and Peter Setlow and Bing Hao},
  journal={Journal of bacteriology},
  year={2013},
  volume={195 11},
  pages={2530-40}
}
Germination of Bacillus spores requires degradation of a modified layer of peptidoglycan (PG) termed the spore cortex by two redundant cortex-lytic enzymes (CLEs), CwlJ and SleB, plus SleB's partner protein, YpeB. In this study, in vitro and in vivo analyses have been used to clarify the roles of individual SleB and YpeB domains in PG degradation. Purified mature Bacillus cereus SleB without its signal sequence (SleB(M)) and the SleB C-terminal catalytic domain (SleB(C)) efficiently triggered… CONTINUE READING