Activity and inhibitor sensitivity of ATPases in the hydrothermal vent tubeworm Riftia pachyptila : a comparative approach

  title={Activity and inhibitor sensitivity of ATPases in the hydrothermal vent tubeworm Riftia pachyptila : a comparative approach},
  author={Shana K Goffredi and James J. Childress},
  journal={Marine Biology},
Abstract Phosphorylated ATPases may be involved in the effective pH regulation seen in the hydrothermal vent tubeworm Riftia pachyptila. R. pachyptila appears not only to have a large concentration of ATPases, but the main function of these ATPases seems to have shifted from other types of transport, such as Na+ and K+ movement, to the facilitation of H+ elimination. Plume and trophosome ATPase activity for R. pachyptila measured 646.2 ± 29.5 and 481.4 ± 32.0 μmol Pi (inorganic phosphate) g−1… Expand
Expression and localization of carbonic anhydrase and ATPases in the symbiotic tubeworm Riftia pachyptila
The expression pattern of cytosolic CA is described using an RNA probe and its histochemical and immunocytochemical localization in the trophosome and branchial plume of Riftia to speculate on the role of CA in bicarbonate and CO2 interconversion to fuel the symbiotic bacteria. Expand
Comparison of proton-specific ATPase activities in plume and root tissues of two co-occurring hydrocarbon seep tubeworm species Lamellibrachia luymesi and Seepiophila jonesi
This study measured the ATPase activities of plume and root tissues collected from L. luymesi and S. jonesi, and used a combination of inhibitors to determine the relative activities of P- and V-type H+-ATPases. Expand
Isolated bacteriocyte cell suspensions from the hydrothermal-vent tubeworm Riftia pachyptila, a potent tool for cellular physiology in a chemoautotrophic symbiosis
A tool for studying cell processes occurring in a bacteria-containing cell by the dissociation of trophosome cell types is described, suggesting the occurrence of potential cytosolic and membrane-associated carbonic anhydrase isoforms in the bacteriocytes, proton-driven sodium-ATPases and a well represented anion transporter exchanging intracellular chloride against extracellular anions. Expand
Comparison of enzyme activities linked to acid–base regulation in a deep-sea and a sublittoral decapod crab species
The hypothesis that the deep-sea Tanner crab has a reduced capacity for active transport of acid-base relevant ions, particularly at the gill, and is therefore at a marked advantage with respect to iono- and acid- base regulatory capacity is supported. Expand
Linking Hydrothermal Geochemistry to Organismal Physiology: Physiological Versatility in Riftia pachyptila from Sedimented and Basalt-hosted Vents
Data from shipboard physiological studies and global quantitative proteomic analyses of Riftia pachyptila trophosome tissue recovered from tubeworms residing in the EPR and the Guaymas basin, a sedimented, hydrothermal vent field, reveal that Riftia symbionts are far more physiologically plastic than previously considered. Expand
Effects of metabolite uptake on proton-equivalent elimination by two species of deep-sea vestimentiferan tubeworm, Riftia pachyptila and Lamellibrachia cf luymesi: proton elimination is a necessary adaptation to sulfide-oxidizing chemoautotrophic symbionts.
It is suggested that protons are the primary waste product of the symbioses of R. pachyptila and L. cf luymesi, and that proton elimination is driven by symbiont metabolism, and may be the largest energetic cost incurred by the worms. Expand
The metabolic demands of endosymbiotic chemoautotrophic metabolism on host physiological capacities
It is shown that moderate to high rates of chemoautotrophic or methanotrophic metabolism impose oxygen uptake and proton equivalent elimination demands upon the hosts that are much higher than is typical for the non-symbiotic annelid, bivalve and gastropod lineages to which they are related. Expand
The hydrocarbon seep tubeworm Lamellibrachia luymesi primarily eliminates sulfate and hydrogen ions across its roots to conserve energy and ensure sulfide supply
It is suggested that L. luymesi has anion exchangers that mediate sulfate elimination coupled with bicarbonate uptake, and Roots could be the ideal exchange surface for eliminating sulfate and hydrogen ions for two reasons. Expand
CO2 Uptake and Fixation by Endosymbiotic Chemoautotrophs from the Bivalve Solemya velum
The reliance of S. velum symbionts on extracellular CO2 is consistent with their intracellular lifestyle, since HCO3− utilization would require protein-mediated transport across the bacteriocyte membrane, perisymbiont vacuole membrane, and symbiont outer and inner membranes. Expand
How to get into bones: proton pump and carbonic anhydrase in Osedax boneworms
Osedax roots have massive acid-secreting capacity via VHA, fuelled by H+ derived from the CA-catalysed hydration of CO2 produced by aerobic metabolism, and it is proposed the secreted acid dissolves the bone carbonate matrix to then allow the absorption of bone-derived nutrients across the skin. Expand