Active site titration and aminoacyl adenylate binding stoichiometry of aminoacyl-tRNA synthetases.

@article{Fersht1975ActiveST,
  title={Active site titration and aminoacyl adenylate binding stoichiometry of aminoacyl-tRNA synthetases.},
  author={A. R. Fersht and J S Ashford and Celia J. Bruton and Ross Jakes and Gordon L. E. Koch and Brian S. Hartley},
  journal={Biochemistry},
  year={1975},
  volume={14 1},
  pages={1-4}
}
A simple, rapid, and economical procedure is described for the determination of the number of catalytically competent active sites on aminoacyl-tRNA synthetases based on the stoichiometry of aminoacyl adenylate formation. On mixing tRNA synthetase, cognate amino acid, (gamma-32P)ATP, and inorganic pyrophosphatase under suitable conditions there is an… CONTINUE READING