Active site-specific immunoassays.

  title={Active site-specific immunoassays.},
  author={Kenneth G. Mann and Edwin B. Williams and Sriram Krishnaswamy and Wynona Church and Alan R. Giles and Russell P Tracy},
  volume={76 4},
This study describes a process by which serine proteases that contain an S-1 arginine subsite and active site histidine may be inactivated and subsequently quantitated using a combination of peptidyl chloromethylketone chemistry and immune recognition technology. Active site labeling and inactivation of proteases is attained by modification of the active site histidine with a peptidyl chloromethylketone. In the specific illustrations demonstrated, we used the compound biotinyl-epsilon… CONTINUE READING