Active site proton delivery and the lyase activity of human CYP17A1.

@article{Khatri2014ActiveSP,
  title={Active site proton delivery and the lyase activity of human CYP17A1.},
  author={Yogan Khatri and Michael C. Gregory and Yelena V. Grinkova and Ilia G Denisov and Stephen G Sligar},
  journal={Biochemical and biophysical research communications},
  year={2014},
  volume={443 1},
  pages={
          179-84
        }
}
  • Yogan Khatri, Michael C. Gregory, +2 authors Stephen G Sligar
  • Published 2014
  • Medicine, Chemistry
  • Biochemical and biophysical research communications
    • Cytochrome P450 CYP17A1 catalyzes a series of reactions that lie at the intersection of corticoid and androgen biosynthesis and thus occupies an essential role in steroid hormone metabolism. This multifunctional enzyme catalyzes the 17α-hydroxylation of Δ4- and Δ5-steroids progesterone and pregnenolone to form the corresponding 17α-hydroxy products through its hydroxylase activity, and a subsequent 17,20-carbon-carbon scission of pregnene-side chain produce the androgens androstenedione (AD… CONTINUE READING
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    Mechanism of Cytochrome P450 17A1-Catalyzed Hydroxylase and Lyase Reactions

    Protein Engineering of the Progesterone Hydroxylating P450-Monooxygenase CYP17A1 Alters Its Regioselectivity.

    Active Site Structures of CYP11A1 in the Presence of Its Physiological Substrates and Alterations upon Binding of Adrenodoxin.

    Human P450 CYP17A1: Control of Substrate Preference by Asparagine 202.

    Structural insights into the function of steroidogenic cytochrome P450 17A1

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    Evidence that cytochrome b5 acts as a redox donor in CYP17A1 mediated androgen synthesis.

    Metabolism of Oral Turinabol by Human Steroid Hormone–Synthesizing Cytochrome P450 Enzymes

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    CITES METHODS

    Kinetic processivity of the two-step oxidations of progesterone and pregnenolone to androgens by human cytochrome P450 17A1

    Cytochrome b5 enhances androgen synthesis by rapidly reducing the CYP17A1 oxy-complex in the lyase step.

    Mechanism of 17α,20-Lyase and New Hydroxylation Reactions of Human Cytochrome P450 17A1

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