Active site localization in a viral mRNA capping enzyme.

@article{Fausnaugh1990ActiveSL,
  title={Active site localization in a viral mRNA capping enzyme.},
  author={J Fausnaugh and A. J. Shatkin},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 13},
  pages={7669-72}
}
Capping of reovirus mRNAs is catalyzed by a guanylyltransferase that corresponds to virion structural polypeptide lambda 2. It forms a phosphoamide linked enzyme-pG covalent complex as an intermediate in the capping reaction. The nucleotide attachment site on lambda 2 was localized to a region between amino acids 213 and 269 by incubating virus particles with [alpha-32P]GTP followed by proteolytic cleavage and analysis of the resulting fragments using sequence-directed antibodies as probes. The… CONTINUE READING

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