Active center rearrangement in RNA polymerase initiation complex.

  title={Active center rearrangement in RNA polymerase initiation complex.},
  author={Arkadv Mustaev and Mikhail Kashlev and Eugeny Zaychikov and Mikhail Aleksandrovich Grachev and A. D. Goldfarb},
  journal={The Journal of biological chemistry},
  volume={268 26},
His1237 in the beta subunit of Escherichia coli RNA polymerase marks the "5' face" of the active center since it can be cross-linked to the gamma-phosphate of the priming substrate. It is demonstrated that RNA chains up to 9 nucleotides in length can be synthesized using His1237-cross-linked nucleotide as a primer. Thus, a substantial mass of RNA can be accommodated in the active center between His1237 and the site of catalysis that remains juxtaposed to the growing 3' end. The apparent… CONTINUE READING