Active B12: a rapid, automated assay for holotranscobalamin on the Abbott AxSYM analyzer.

  title={Active B12: a rapid, automated assay for holotranscobalamin on the Abbott AxSYM analyzer.},
  author={Jeff Brady and Lesley Wilson and Lynda McGregor and Edward Valente and Lars Orning},
  journal={Clinical chemistry},
  volume={54 3},
BACKGROUND Conventional tests for vitamin B(12) deficiency measure total serum vitamin B12, whereas only that portion of vitamin B12 carried by transcobalamin (holotranscobalamin) is metabolically active. Measurement of holotranscobalamin (holoTC) may be more diagnostically accurate for detecting B(12) deficiency that requires therapy. We developed an automated assay for holoTC that can be used on the Abbott AxSYM immunoassay analyzer. METHODS AxSYM Active B12 is a 2-step sandwich… CONTINUE READING


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