Activation tagging approach in a model legume, Lotus japonicus

  title={Activation tagging approach in a model legume, Lotus japonicus},
  author={Ryujiro Imaizumi and Shusei Sato and Nanako Kameya and Ikuo Nakamura and Yasukazu Nakamura and Satoshi Tabata and Shin-ichi Ayabe and Toshio Aoki},
  journal={Journal of Plant Research},
We constructed T-DNA insertional lines of a model legume, Lotus japonicus, using a multifunctional vector for gene and exon activation tagging. The vector had the CaMV 35S promoter together with two additional enhancer elements, the start codon, and splice donor and acceptor sites facing the left border, in anticipation of the activation of T-DNA flanking genes and forced expression of flanking exons. The improved transformation technique yielded more than 3,500 lines, including 45 dominant… CONTINUE READING
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