Activation of the thyrotropin-releasing hormone (TRH) receptor by a direct precursor of TRH, TRH-Gly

@article{Yamada1995ActivationOT,
  title={Activation of the thyrotropin-releasing hormone (TRH) receptor by a direct precursor of TRH, TRH-Gly},
  author={Masanobu Yamada and Toshiharu Iwasaki and Teturo Satoh and Tsuyoshi Monden and Shuntaro Konaka and M. Murakami and Tokuji Iriuchijima and Masatomo Mori},
  journal={Neuroscience Letters},
  year={1995},
  volume={196},
  pages={109-112}
}
We studied the mechanism by which thyrotropin-releasing hormone (TRH)-Gly stimulated prolactin and thyrotropin (TSH) secretion in pituitary, using a pituitary mammotropic cell line, GH3 cells, and a cell line stably expressing a human TRH receptor (TRH-R). In GH3 cells expressing endogenous TRH-R, an addition of TRH-Gly evoked an immediate rise of intracellular calcium concentration, indicating that TRH-Gly reacted directly without converting from TRH-Gly to TRH. In order to determine whether… Expand
Expression of thyrotropin-releasing hormone (TRH) receptor subtype 1 in mouse pancreatic islets and HIT-T15, an insulin-secreting clonal beta cell line.
TLDR
This is the first description indicating the presence of functional TRH receptor subtype 1 in the pancreatic beta cells, and the observations suggested the regulation of pancreatic function by TRH through autocrine or paracrine mechanisms. Expand
Thyrotropin-releasing hormone (TRH) in the cerebellum
TLDR
THyrotropin-releasing hormone and TRH analogs are promising clinical therapeutic agents for inducing arousal effects, amelioration of mental depression, and improvement of cerebellar ataxia. Expand
A novel transcript for the thyrotropin-releasing hormone receptor in human pituitary and pituitary tumors.
TLDR
Findings indicate that this novel transcript of the human TRH receptor gene is produced in a tumor-specific manner and may be a useful parameter for evaluation of individual pituitary tumors. Expand
The role of the thyrotropin-releasing hormone (TRH) neuron as a metabolic sensor.
  • A. Hollenberg
  • Biology, Medicine
  • Thyroid : official journal of the American Thyroid Association
  • 2008
TLDR
By integrating these inputs, the TRH neuron is able to set the thyroid axis at the appropriate level given the physiologic demands present. Expand
The biology of pro-thyrotropin-releasing hormone-derived peptides.
TLDR
I. Biosynthesis of TRH and Other pro-TRH-Derived Peptide Receptors, and the role of PCs and CPs and Neuropeptide and catecholamine regulation of Pro- TRH biosynthesis and processing. Expand
The Thyroid Hormone Axis: Its Roles in Body Weight Regulation, Obesity, and Weight Loss
TLDR
In humans and other vertebrates, thyroid hormone is essential to the development and function of every tissue in the body and is a poor therapeutic to treat obesity and to elevate energy expenditure. Expand
Biosynthesis of proTRH-derived peptides in prohormone convertase 1 and 2 knockout mice
Prohormone convertases (PCs) 1 and 2 are the primary endoproteases involved in the post-translational processing of proThyrotropin Releasing Hormone (proTRH) to give rise to TRH and other proposedExpand
Pituitary cell lines and their endocrine applications
TLDR
The morphology and function of the cell types that make up the anterior pituitary, and the characteristics of the various functional anterior Pituitary cell systems that have been established to be representative of each anterior pituitsary cell lineage are discussed. Expand
A new endogenous form of PYY isolated from canine ileum: Gly-extended PYY(1-36)
TLDR
It is found that the PYY(3-36)-NH(2) form represents 6% of the total PYY in the canine ileo-cecal junction, and the physiological implication of the Gly-extended form of PYY (1-36) warrants further investigation. Expand
The purification and characterisation of a membrane bound pyroglutamyl aminopeptidase type-II from bovine brain
Three new fluonmetnc assays were developed for the detection of pyroglutamyl ammopeptidase type-II (PAPII) activity. Two of these assays are based on hydrolysis of the TRH substrate analogueExpand
...
1
2
...

References

SHOWING 1-10 OF 16 REFERENCES
Release of thyrotropin and prolactin by a thyrotropin-releasing hormone (TRH) precursor, TRH-Gly: conversion to TRH is sufficient for in vivo effects.
TLDR
The mechanism by which TRH-Gly stimulates pituitary thyrotropin (TSH) and prolactin release has been studied in urethane-anesthetized 2-month-old male Sprague-Dawley rats and in vitro with GH3 cells, a rat anterior pituitARY tumor cell line and the binding of TRH/Gly to the plasma membrane receptors for TRH onGH3 cells was investigated. Expand
Thyroid hormone modulation of thyrotrophin-releasing hormone (TRH) and TRH-Gly levels in the male rat reproductive system.
TLDR
It is demonstrated that in the male rat reproductive system the levels of TRH and its immediate biosynthetic precursor, TRH-Gly, are regulated by thyroid hormones. Expand
Stimulation by a TRH precursor, TRH-Gly, of TSH and PRL secretion in rats: Effect of starvation
TLDR
In starved rats, TRH-Gly apparently stimulated TSH and PRL secretion in a dose-dependent manner, and the stimulatory activity increased in starved rats as compared to normal controls, implying that large amounts of TRH -Gly may have significant biological activities and these are potentiated in the starved condition. Expand
Distribution of thyrotropin-releasing hormone (TRH) and precursor peptide (TRH-Gly) in adult rat tissues.
TLDR
The results suggest that 1) the processing of TRH-Gly to TRH varies among tissues, 2) TRH/Gly/TRH conversion occurs most efficiently in neural tissues, and 3) TR h/gly/ TRH conversion may be a rate-limiting step in TRH biosynthesis. Expand
Pituitary adenomas of patients with acromegaly express thyrotropin-releasing hormone receptor messenger RNA: cloning and functional expression of the human thyrotropin-releasing hormone receptor gene.
TLDR
The sequence and functioning analyses showed the same expression of TRH-R mRNA in the pituitary adenomas of patients with acromegaly as that in the normal pituitaries, indicating that 2) the paradoxical response of GH secretion to TRH found in the patients might be a direct effect ofTRH on TRH -R expressed in the adenoma. Expand
Differential regulation of thyrotropin-releasing hormone receptor mRNA levels by thyroid hormone in vivo and in vitro (GH3 cells).
TLDR
The present data indicate that the in vivo effects of thyroid status on TRH-R mRNA levels differ from the in vitro one, and that the down regulation of TRh-R binding by thyroid hormone in GH3 cells may be mediated by translational or post-translational mechanisms. Expand
Thyrotropin-releasing hormone precursor: characterization in rat brain.
TLDR
Findings indicate that mammalian TRH arises by posttranslational processing of a larger precursor protein, and the ability of the TRH prohormone to generate multiple copies of the bioactive peptide may be an important mechanism in the amplification of hormone production. Expand
High levels of thyrotropin-releasing hormone precursor peptide immunoreactivity and binding substance occur in human cerebrospinal fluid.
TLDR
The TRH-Gly RIA was used to determine whether this TRH precursor peptide is also elevated in CSF from MS and Alzheimer's disease patients in comparison with the corresponding levels in non-central nervous system disease (control) patients. Expand
Thyroid hormone modulation of TRH precursor levels in rat hypothalamus, pituitary, thyroid and blood
TLDR
The ratio of TRH-Gly to TRH was significantly increased in the anterior pituitary by hypothyroidism andTRH injection, suggesting that thyroid hormones and TRH regulate the alpha-amidation of TRh-Gy to form TRH in this tissue. Expand
A possible direct precursor of thyrotropin-releasing hormone, pGlu-His-Pro-Gly, stimulates prolactin secretion in anorexia nervosa.
TLDR
Evidence is provided that PRL secretion in anorectic patients is quantitatively different from that in normal persons, and TRH-Gly did not alter PRL levels in patients with uremia, acromegaly, or prolactinoma. Expand
...
1
2
...