Activation of the prereplication complex is blocked by mimosine through reactive oxygen species-activated ataxia telangiectasia mutated (ATM) protein without DNA damage.

@article{Kubota2014ActivationOT,
  title={Activation of the prereplication complex is blocked by mimosine through reactive oxygen species-activated ataxia telangiectasia mutated (ATM) protein without DNA damage.},
  author={Shoichi Kubota and Yasunori Fukumoto and Kenichi Ishibashi and Shuhei Soeda and Sho Kubota and Ryuzaburo Yuki and Yuji Nakayama and Kazumasa Aoyama and Noritaka Yamaguchi and Naoto Yamaguchi},
  journal={The Journal of biological chemistry},
  year={2014},
  volume={289 9},
  pages={
          5730-46
        }
}
Mimosine is an effective cell synchronization reagent used for arresting cells in late G1 phase. However, the mechanism underlying mimosine-induced G1 cell cycle arrest remains unclear. Using highly synchronous cell populations, we show here that mimosine blocks S phase entry through ATM activation. HeLa S3 cells are exposed to thymidine for 15 h, released for 9 h by washing out the thymidine, and subsequently treated with 1 mM mimosine for a further 15 h (thymidine → mimosine). In contrast to… CONTINUE READING

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