Activation of the kexin from Schizosaccharomyces pombe requires internal cleavage of its initially cleaved prosequence.

@article{Powner1998ActivationOT,
  title={Activation of the kexin from Schizosaccharomyces pombe requires internal cleavage of its initially cleaved prosequence.},
  author={Dale J. Powner and John Davey},
  journal={Molecular and cellular biology},
  year={1998},
  volume={18 1},
  pages={400-8}
}
Members of the kexin family of processing enzymes are responsible for the cleavage of many proproteins during their transport through the secretory pathway. The enzymes themselves are made as inactive precursors, and we investigated the activation process by studying the maturation of Krp1, a kexin from the fission yeast Schizosaccharomyces pombe. Using a cell-free translation-translocation system prepared from Xenopus eggs, we found that Krp1 is made as a preproprotein that loses the… CONTINUE READING