Activation of the dynein adenosinetriphosphatase by microtubules.

  title={Activation of the dynein adenosinetriphosphatase by microtubules.},
  author={Charlotte K. Omoto and K A Johnson},
  volume={25 2},
Previous work has indicated that following the rapid adenosine 5'-triphosphate (ATP) induced dissociation of the microtubule-dynein complex, the rate-limiting step in the ATPase cycle is product release [Johnson, K. A. (1983) J. Biol. Chem. 258, 13825-13832], which occurs at a rate of approximately 2-6 s-1. In this report we complete the analysis of the ATPase cycle by examining the effect of microtubules on the rate of product release. For these studies we used repolymerized Tetrahymena… CONTINUE READING