Activation of the Small G Protein Arf6 by Dynamin2 through Guanine Nucleotide Exchange Factors in Endocytosis

@inproceedings{Okada2015ActivationOT,
  title={Activation of the Small G Protein Arf6 by Dynamin2 through Guanine Nucleotide Exchange Factors in Endocytosis},
  author={Risa Okada and Yohei Yamauchi and Tsunaki Hongu and Yuji Funakoshi and Norihiko Ohbayashi and Hiroshi Hasegawa and Yasunori Kanaho},
  booktitle={Scientific reports},
  year={2015}
}
The small G protein Arf6 and the GTPase dynamin2 (Dyn2) play key roles in clathrin-mediated endocytosis (CME). However, their functional relationship remains obscure. Here, we show that Arf6 functions as a downstream molecule of Dyn2 in CME. Wild type of Dyn2 overexpressed in HeLa cells markedly activates Arf6, while a GTPase-lacking Dyn2 mutant does not. Of the Arf6-specific guanine nucleotide exchange factors, EFA6A, EFA6B, and EFA6D specifically interact with Dyn2. Furthermore… CONTINUE READING