Activation of the Nipah virus fusion protein in MDCK cells is mediated by cathepsin B within the endosome-recycling compartment.

@article{Diederich2012ActivationOT,
  title={Activation of the Nipah virus fusion protein in MDCK cells is mediated by cathepsin B within the endosome-recycling compartment.},
  author={Sandra Diederich and Lucie Sauerhering and Michael C. Weis and Hermann Altmeppen and Norbert Schaschke and Thomas Reinheckel and Stephanie Erbar and Andrea Maisner},
  journal={Journal of virology},
  year={2012},
  volume={86 7},
  pages={3736-45}
}
Proteolytic activation of the fusion protein of the highly pathogenic Nipah virus (NiV F) is a prerequisite for the production of infectious particles and for virus spread via cell-to-cell fusion. Unlike other paramyxoviral fusion proteins, functional NiV F activation requires endocytosis and pH-dependent cleavage at a monobasic cleavage site by endosomal proteases. Using prototype Vero cells, cathepsin L was previously identified to be a cleavage enzyme. Compared to Vero cells, MDCK cells… CONTINUE READING