Activation of the MCM2-7 helicase by association with Cdc45 and GINS proteins.

@article{Ilves2010ActivationOT,
  title={Activation of the MCM2-7 helicase by association with Cdc45 and GINS proteins.},
  author={Ivar Ilves and Tatjana Petojevic and James J Pesavento and Michael R Botchan},
  journal={Molecular cell},
  year={2010},
  volume={37 2},
  pages={
          247-58
        }
}
MCM2-7 proteins provide essential helicase functions in eukaryotes at chromosomal DNA replication forks. During the G1 phase of the cell cycle, they remain loaded on DNA but are inactive. We have used recombinant methods to show that the Drosophila MCM2-7 helicase is activated in complex with Cdc45 and the four GINS proteins (CMG complex). Biochemical activities of the MCM AAA+ motor are altered and enhanced through such associations: ATP hydrolysis rates are elevated by two orders of magnitude… CONTINUE READING
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