Activation of the IκB Kinase Complex by TRAF6 Requires a Dimeric Ubiquitin-Conjugating Enzyme Complex and a Unique Polyubiquitin Chain
@article{Deng2000ActivationOT, title={Activation of the I$\kappa$B Kinase Complex by TRAF6 Requires a Dimeric Ubiquitin-Conjugating Enzyme Complex and a Unique Polyubiquitin Chain}, author={Li Deng and chen wang and Erika Spencer and Liyong Yang and A. Braun and Jianxin You and Clive Slaughter and Cecile M. Pickart and Zhijian J. Chen}, journal={Cell}, year={2000}, volume={103}, pages={351-361} }
1,805 Citations
TIFA activates IkappaB kinase (IKK) by promoting oligomerization and ubiquitination of TRAF6.
- BiologyProceedings of the National Academy of Sciences of the United States of America
- 2004
Evidence is presented that TIFA (TRAF-interacting protein with a forkhead-associated domain) activates IKK by promoting the oligomerization and Ub ligase activity of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism.
TAK1 is a ubiquitin-dependent kinase of MKK and IKK
- BiologyNature
- 2001
The purification and identification of TRIKA2, which is composed of TAK1, TAB1 and TAB2, a protein kinase complex previously implicated in IKK activation through an unknown mechanism, indicate that ubiquitination has an important regulatory role in stress response pathways, including those of IKK and JNK.
TRAF6 Autoubiquitination-Independent Activation of the NFκB and MAPK Pathways in Response to IL-1 and RANKL
- BiologyPloS one
- 2008
While TRAF6 autoubiquitination may serve as a marker of activation, it is unlikely to underpin RING finger-dependent TRAF 6 function.
Direct Activation of Protein Kinases by Unanchored Polyubiquitin Chains
- Biology, ChemistryNature
- 2009
It is shown that free Lys 63 polyubiquitin chains, which are not conjugated to any target protein, directly activate TAK1 by binding to the ubiquitin receptor TAB2 (also known as MAP3K7IP2), suggesting a new mechanism of protein kinase regulation.
TRAF2: A Double-Edged Sword?
- BiologyScience's STKE
- 2005
Ubiquitination is best known for its role in targeting proteins for degradation by the proteasome, but evidence of the nonproteolytic functions of ubiquitin is also rapidly accumulating. One example…
Molecular basis for the unique deubiquitinating activity of the NF-kappaB inhibitor A20.
- BiologyJournal of molecular biology
- 2008
Mechanism of ubiquitin transfer promoted by TRAF6
- BiologyProceedings of the National Academy of Sciences
- 2018
Through structural modeling and biochemical analysis, it is shown that the TRAF6 RING dimer employs a concerted allosteric mechanism using both subunits of the TRAf6 dimer to promote Ub transfer through engagement with a donor-Ub in cis and in trans, respectively.
Role of K63-linked polyubiquitination in NF-κB signalling: which ligase catalyzes and what molecule is targeted?
- BiologyJournal of biochemistry
- 2012
A cell free IKK assay system is established and demonstrated that recombinant Tax protein can activate the IKK complex in a K63-linked-polyubiquitination-dependent manner, useful for the identification of various key players responsible for Tax-induced IKK activation.
The coiled-coil domain of TRAF6 is essential for its auto-ubiquitination.
- BiologyBiochemical and biophysical research communications
- 2004
Immunoblot analysis of linear polyubiquitination of NEMO.
- BiologyMethods in molecular biology
- 2015
The protocol for the detection of the linear polyubiquitination of NEMO by the immunoblotting using anti-linear ubiquitin antibody is described.
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