Activation of receptor tyrosine kinases promotes gene transfection in rat neuronal PC12 cells by cationic liposomes

  title={Activation of receptor tyrosine kinases promotes gene transfection in rat neuronal PC12 cells by cationic liposomes},
  author={Ari Noguchi and Naohide Hirashima and Tadahide Furuno and Mamoru Nakanishi},
  journal={Neuroscience Letters},
Cationic liposomes are commonly used to introduce foreign genes to the target cells. However, the methods are not applicable to several types of cells such as neuronal cells. We introduced the luciferase gene to the neuronal pheochromocytoma-12 (PC12) cells by cationic liposomes with a cationic cholesterol derivative (I) or 3- beta - [N - (N',N' - dimethylaminoethane) - carbamoyl]- cholesterol (DC - Chol). Efficiency of gene transfection into undifferentiated naive PC12 cells was extremely… 
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Remarkable induction of apoptosis in cancer cells by a novel cationic liposome complexed with a bcl-2 antisense oligonucleotide.
The results showed the possibility that this new cationic cholesterol derivative might be very promising to be used for liposome-mediated gene targeting in vitro and in vivo.
Asialoganglioside enhances the efficiency of gene transfection mediated by cationic liposomes with a cationic cholesterol derivative.
Together with the results obtained by luciferase assay, the removal of sialic acid from ganglioside causes the enhancement of efficiency of transfection mediated by cationic liposomes.
Characterization of biosurfactant-containing liposomes and their efficiency for gene transfection.
The results indicated that these kinds of the physicochemical properties in MEL-A-containing liposomes are able to increase the efficiency of liposome-mediated gene transfection.
An epoch-making application of discharge plasma phenomenon to gene-transfer.
The plasma method is an epoch-making gene transfer technology which efficiently transfers genes into primary cells into which electroporation cannot transfer genes, and is able to universally transfer genes into various types of cells as the function of the cells was maintained.
Biosurfactant MEL-A dramatically increases gene transfection via membrane fusion.
The results obtained by CLSM images were in good agreement with the transfection efficiency, which suggests that MEL-A induces the membrane fusion between the target cells and the cationic liposomes, accelerating the efficiency of gene transfections dramatically.


Membrane fusion plays an important role in gene transfection mediated by cationic liposomes
The results suggest that at least two steps are effective for gene transfection mediated by the cationic liposomes with cATIONic cholesterol derivatives, including the endocytosis of the liposome/DNA complex into the target cells and the removal of antisense oligonucleotides (plasmid DNAs) from the complex in the endosomes.
Effect of cationic cholesterol derivatives on gene transfer and protein kinase C activity.
Four different cationic derivatives of cholesterol were synthesized which contain either a tertiary or a quaternary amino head group, with and without a succinyl spacer-arm, and several adherent cell lines could be efficiently transfected with this liposome reagent without any apparent cytotoxicity.
Confocal and probe microscopy to study gene transfection mediated by cationic liposomes with a cationic cholesterol derivative.
A novel cationic cholesterol derivative with a hydroxyethyl amino head group (I) has been synthesized and used for liposome-mediated gene transfection and it was found that microtubules were involved in the intracellular dynamics of genetransfection.
Cationic cholesterol with a hydroxyethylamino head group promotes significantly liposome‐mediated gene transfection
A novel cationic cholesterol derivative with a hydroxyethylamino head group, cholesteryl‐3β‐carboxyamidoethylene‐N‐hydroxyethylamine (II), has been synthesized and used for liposome‐mediated gene transfection and facilitated greatly pSV2CAT gene transference into mouse NIH3T3 and L929 cells in the absence of serum.
The trk proto-oncogene encodes a receptor for nerve growth factor
The results indicate that gp140trk is a functional NGF receptor that mediates at least some of the signal transduction processes initiated by this neurotrophic factor.
Cationic lipids, phosphatidylethanolamine and the intracellular delivery of polymeric, nucleic acid-based drugs (review).
Most evidence now supports the hypothesis that lipid-DNA drugs enter target cells by endocytosis and disrupt the endosomal membrane, releasing nucleic acid into the cytoplasm, and the challenges that remain in bringing nucleic acids based drugs into the realm of clinical reality are considered.
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
Depending upon the cell line, lipofection is from 5- to greater than 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.
A novel cationic liposome reagent for efficient transfection of mammalian cells.
  • X. Gao, L. Huang
  • Chemistry, Medicine
    Biochemical and biophysical research communications
  • 1991
A novel cationic derivative of cholesterol, 3 beta [N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol), has been synthesized and used to prepare sonicated liposomes with dioleoylphosphatidylethanolamine and facilitates efficient DNA mediated transfection in A431 human epidermoid carcinoma cells.
Effect of zeta potential of cationic liposomes containing cationic cholesterol derivatives on gene transfection
Eight cationic derivatives of cholesterol which contain a tertiary amino head group with a different spacer arm are synthesized and indicated that zeta potentials of cationIC liposomes were well related to transfection activity of pSV2CAT DNA.
PC12 pheochromocytoma cells: culture, nerve growth factor treatment, and experimental exploitation.
Methods for the maintenance and nerve growth factor (NGF) treatment of PC12 cultures as well as for the generation and selection of PC 12 cell mutants and for the preparation of a PC12 cell fraction, which is highly enriched in neurites are presented.