Activation of pyruvate oxidase by monomeric and micellar amphiphiles.

Pyruvate oxidase is a peripheral membrane enzyme that can be purified to homogeneity in the absence of detergents. Once removed from the membrane particulate fraction of the cell, the activity of the oxidase in a dye-mediated assay is stimulated ZOto 50-fold by incubation with a wide variety of amphiphiles. Over 120 amphiphilic compounds have been screened… CONTINUE READING