Activation of proteinase-activated receptor-1 inhibits neurally evoked chloride secretion in the mouse colon in vitro.

@article{Buresi2005ActivationOP,
  title={Activation of proteinase-activated receptor-1 inhibits neurally evoked chloride secretion in the mouse colon in vitro.},
  author={Michelle Buresi and Nathalie Vergnolle and Keith A Sharkey and Catherine M. Keenan and Patricia Andrade-Gordon and Giuseppe Antonio Cirino and Donatella Cirillo and Morley D Hollenberg and Wallace K MacNaughton},
  journal={American journal of physiology. Gastrointestinal and liver physiology},
  year={2005},
  volume={288 2},
  pages={G337-45}
}
The proteinase-activated thrombin receptor-1 (PAR-1) belongs to a unique family of G protein-coupled receptors activated by proteolytic cleavage. We studied the effect of PAR-1 activation in the regulation of ion transport in mouse colon in vitro. Expression of PAR-1 in mouse colon was assessed by RT-PCR and immunohistochemistry. To study the role of PAR-1 activation in chloride secretion, mouse colon was mounted in Ussing chambers. Changes in short-circuit current (Isc) were measured in… CONTINUE READING